kmerApproach.snk 18.1 KB
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# Returns the correct filename in which the required information is stored depending on the configuration setting
def determineKmerCoverageEstimateFile():
    if config['kmerCoverageEstimationMethod'] == 'alignment':
        return 'data/auxiliary/'+config['input_folder']+'/kmers/{kmer}/{id}/coverage_estimate_alignmentbased.txt'
    elif config['kmerCoverageEstimationMethod'] == 'countMean':
        return 'data/auxiliary/'+config['input_folder']+'/kmers/{kmer}/{id}/coverage_estimate_kmercountbased.txt'
    elif config['kmerCoverageEstimationMethod'] == 'countPoisson':
        return 'data/auxiliary/'+config['input_folder']+'/kmers/{kmer}/{id}/coverage_estimate_kmercountbased.txt'

# Returns the corresponding ground truth spa-type for a given file (sample) id
def getGroundTruthType(fid):
    with open('data/input/groundTruth.tsv','r') as gtfile:

        for l in gtfile.read().splitlines():
            data = l.split('\t')
            if data[0] == fid:
                return data[1]
        else:
            raise WorkflowError("Can't find {} in ground truth file ... check that an entry exists!".format(fid))

### Mapping ###

rule extractMaskedReferenceGenome:
    input:
        refg = 'data/input/'+config['reference_genome']+'/'+config['genome_file_identifier'],
        pt = 'data/input/'+config['reference_genome']+'/'+config['protein_table_identifier']
    output:
        main = 'data/auxiliary/maskedRef.fa'
    params:
        pid = config['protein_a_identifier'],
        #cluster execution
        cpus = '1',
        mem = '8G',
        gpus = '0',
        walltime = '00:05:00'
    conda:
        '../envs/biopythonworkbench.yaml'
    script:
        '../scripts/maskProteinA.py'

rule createSyntheticProteinAs:
    input:
        proteinAFrame = 'data/input/proteinAFrame.ref',
        spaSequences = 'data/auxiliary/spaSequences.fa'
    output:
        seqs = 'data/auxiliary/syntheticProteinAs.fa',
        metaInf = 'data/auxiliary/syntheticProteinAs.meta'
    params:
        #cluster execution
        cpus = '1',
        mem = '8G',
        gpus = '0',
        walltime = '00:05:00'
    conda:
        '../envs/biopythonworkbench.yaml'
    script:
        '../scripts/createSyntheticProteinAs.py'
        
rule concatRelevantSequences:
    input:
        synth = 'data/auxiliary/syntheticProteinAs.fa',
        masked = 'data/auxiliary/maskedRef.fa'
    output:
        'data/auxiliary/matchBoard.fa'
    params:
        #cluster execution
        cpus = '1',
        mem = '8G',
        gpus = '0',
        walltime = '00:02:00'
    shell:
        'cat {input.masked} {input.synth} > {output}'
        
rule bwa:
    input:
        bwi = 'data/auxiliary/matchBoard.fa.bwt',
        mb = 'data/auxiliary/matchBoard.fa',
        read1 = 'data/auxiliary/' + config['input_folder'] + '/{id}' + '.qc' + config['input_read_1_ending'],
        read2 = 'data/auxiliary/' + config['input_folder'] + '/{id}' + '.qc' + config['input_read_2_ending']
    output:
        'data/auxiliary/'+config['input_folder']+'/{id}/alignment.bam'
    params:
        #cluster execution
        cpus = '1',
        mem = '32G',
        gpus = '0',
        walltime = '00:30:00'
    singularity:
        'docker://biocontainers/bwa:v0.7.17-3-deb_cv1'
    shell:
        'bwa mem {input.mb} {input.read1} {input.read2} -o {output}'
        #'bwa mem {input.mb} {input.read1} -o {output}'
        


rule determineStrandBias:
    input:
        alignment = 'data/auxiliary/'+config['input_folder']+'/{id}/alignment.sorted.bam',
        idx =	'data/auxiliary/'+config['input_folder']+'/{id}/alignment.sorted.bam.bai'
    output:
        strandbias = report('data/output/'+config['input_folder']+'/{id}/strandbias.txt',category='Strand Bias')
    params:
        #cluster execution
        cpus = '1',
        mem = '32G',
        gpus = '0',
        walltime = '00:30:00'	
    conda:
        '../envs/biopythonworkbench.yaml'
    script:
        '../scripts/determineStrandBias.py'



rule filter_primary_matches:
    input:
        alignment = 'data/auxiliary/'+config['input_folder']+'/{id}/alignment.sorted.bam',
        whitelist = 'data/auxiliary/syntheticProteinAs.meta'
    output:
        'data/auxiliary/'+config['input_folder']+'/{id}/alignment.filtered.bam'
    params:
        #cluster execution
        cpus = '1',
        mem = '16G',
        gpus = '0',
        walltime = '00:10:00'
    conda:
        '../envs/biopythonworkbench.yaml'
    shell:
        'samtools view -F 4 {input.alignment} -L {input.whitelist} -b > {output}'

rule determineFilteredStrandBias:
    input:
        alignment = 'data/auxiliary/'+config['input_folder']+'/{id}/alignment.filtered.bam',
        idx = 'data/auxiliary/'+config['input_folder']+'/{id}/alignment.filtered.bam.bai'
    output:
        strandbias = 'data/auxiliary/'+config['input_folder']+'/{id}/strandbias.filtered.txt'
    params:
        #cluster execution
        cpus = '1',
        mem = '16G',
        gpus = '0',
        walltime = '00:30:00'
    conda:
        '../envs/biopythonworkbench.yaml'
    script:
        '../scripts/determineStrandBias.py'	

rule extractFilteredReadsAsFastQ:
    input:
        filteredAlignment = 'data/auxiliary/'+config['input_folder']+'/{id}/alignment.filtered.bam'
    output:
        filteredReads = 'data/auxiliary/'+config['input_folder']+'/{id}/filteredReads.fastq'
    params:
        #cluster execution
        cpus = '1',
        mem = '8G',
        gpus = '0',
        walltime = '00:10:00'
    conda:
        '../envs/biopythonworkbench.yaml'
    shell:
        'samtools fastq {input.filteredAlignment} > {output.filteredReads}'




rule createKmerDistributionGroundTruth_COVERAGE_BASED:
    input:
        expectedCounts = 'data/auxiliary/'+config['input_folder']+'/kmers/{kmer}/{id}/expected_counts.json',
        observedCounts = 'data/auxiliary/'+config['input_folder']+'/kmers/{kmer}/{id}/alignment.counts.json',
        probabilities = 'data/auxiliary/'+config['input_folder']+'/kmers/{kmer}/{id}/scores.probabilistic_cov.tsv',
        kmerError = 'data/auxiliary/'+config['input_folder']+'/kmers/{kmer}/{id}/kmer_error.txt'
    output:
        errors = 'data/output/'+config['input_folder']+'/methodAnalysis/{kmer}/{id}/kmerErrorDistributions.svg',
        deviations = 'data/output/'+config['input_folder']+'/methodAnalysis/{kmer}/{id}/countDeviations.svg'
    params:
        gt = lambda wildcards : getGroundTruthType(wildcards.id),
        #cluster execution
        cpus = '1',
        mem = '64G',
        gpus = '0',
        walltime = '00:30:00'
    conda:
        '../envs/biopythonworkbench.yaml'
    log:
        'logs/'+config['input_folder']+'/methodAnalysis/{kmer}/{id}/kmerErrorDistributions.svg'
    script:
        '../scripts/createKmerErrorDistributionPlots.py'


rule likelihoodAnalysis_COVERAGE_BASED:
    input:
        expectedCounts = 'data/auxiliary/'+config['input_folder']+'/kmers/{kmer}/{id}/expected_counts.json',
        observedCounts = 'data/auxiliary/'+config['input_folder']+'/kmers/{kmer}/{id}/alignment.counts.json',
        probabilities = 'data/auxiliary/'+config['input_folder']+'/kmers/{kmer}/{id}/scores.probabilistic_cov.tsv',
        kmerError = 'data/auxiliary/'+config['input_folder']+'/kmers/{kmer}/{id}/kmer_error.txt'
    output:
        likelihoodAnalysis = 'data/output/'+config['input_folder']+'/methodAnalysis/{kmer}/{id}/likelihoodAnalysis.txt'
    params:
        gt = lambda wildcards : getGroundTruthType(wildcards.id)
    conda:
        '../envs/biopythonworkbench.yaml'
    log:
        'logs/'+config['input_folder']+'/methodAnalysis/{kmer}/{id}/likelihoodAnalysis.txt'
    script:
        '../scripts/likelihoodBreakdown.py'

rule mapAgainstGroundTruth:
    input:
        filteredReads = 'data/auxiliary/'+config['input_folder']+'/{id}/filteredReads.fastq',
        groundTruthSequence = lambda wildcards: 'data/input/ref/'+getGroundTruthType(wildcards.id)+'.fa',
        groundTruthIndex = lambda wildcards: 'data/input/ref/'+getGroundTruthType(wildcards.id)+'.fa.bwt'
    output:
        'data/output/'+config['input_folder']+'/methodAnalysis/{id}/alignmentToGroundTruthType.bam'
    params:
        # cluster execution
        cpus = '1',
        mem = '64G',
        gpus = '0',
        walltime = '00:30:00'
    singularity:
        'docker://biocontainers/bwa:v0.7.17-3-deb_cv1'
    shell:
        'bwa mem {input.groundTruthSequence} {input.filteredReads} -o {output}'

rule verifyUniqueness:
    input:
        kmerCounts = 'data/auxiliary/kmers/{kmer}/spaSequences.counts.json',
        maskedReference = 'data/auxiliary/maskedRef.fa'
    output:
        report('data/output/kmers/{kmer}/uniquenessTest.tsv',category='kmerUniqueness')
    conda:
        '../envs/biopythonworkbench.yaml'
    params:
        k = lambda wildcards : wildcards.kmer,
        # cluster execution
        cpus = '1',
        mem = '8G',
        gpus = '0',
        walltime = '00:30:00'
    script:
        '../scripts/verifyUniqueness.py'

rule analyzeMapping:
    input:
        read1 = 'data/auxiliary/'+config['input_folder']+'/{id}'+'.qc'+config['input_read_1_ending'],
        read2 = 'data/auxiliary/'+config['input_folder']+'/{id}'+'.qc'+config['input_read_2_ending'],
        filteredAlignment = 'data/auxiliary/'+config['input_folder']+'/{id}/alignment.filtered.bam',
        idx = 'data/auxiliary/'+config['input_folder']+'/{id}/alignment.filtered.bam.bai',
        metaInf = lambda wildcards : 'data/input/'+config['input_folder']+'/syntheticReferencesMetaData/'+getGroundTruthType(wildcards.id)+'.meta'
    output:
        correctAlignments = 'data/output/'+config['input_folder']+'/methodAnalysis/{id}/correctMapping.fa',
        analysis = 'data/output/'+config['input_folder']+'/methodAnalysis/{id}/mapping.comparison'
    conda:
        '../envs/biopythonworkbench.yaml'
    params:
        # cluster execution
        cpus = '1',
        mem = '1G',
        gpus = '0',
        walltime = '00:10:00'
    script:
        '../scripts/analyzeMapping.py'

rule makeKmerProfilesFromTrueReads:
    input:
        filteredReads = 'data/output/'+config['input_folder']+'/methodAnalysis/{id}/correctMapping.fa',
        regionXMetaData = lambda wildcards : 'data/input/'+config['input_folder']+'/syntheticReferencesMetaData/'+getGroundTruthType(wildcards.id)+'.meta'
    output:
        counts = 'data/output/'+config['input_folder']+'/methodAnalysis/{id}/{kmer}/correctCounts.json',
        origins = 'data/output/'+config['input_folder']+'/methodAnalysis/{id}/{kmer}/kmerOrigins.json'
    params:
        k = lambda wildcards: wildcards.kmer
    conda:
        '../envs/biopythonworkbench.yaml'
    script:
        '../scripts/makeKmerProfilesFromTrueReads.py'

rule compareObservedKmerProfileToTrueProfile:
    input:
        trueCounts = 'data/output/'+config['input_folder']+'/methodAnalysis/{id}/{kmer}/correctCounts.json',
        observedCounts = 'data/auxiliary/'+config['input_folder']+'/kmers/{kmer}/{id}/alignment.counts.json'
    output:
        differences = 'data/output/'+config['input_folder']+'/methodAnalysis/{id}/{kmer}/differences_observed.txt'
    conda:
        '../envs/biopythonworkbench.yaml'
    script:
        '../scripts/compareKmerCounts.py'

rule compareExpectedKmerProfileToObserved:
    input:
        trueCounts = 'data/auxiliary/'+config['input_folder']+'/kmers/{kmer}/{id}/alignment.counts.json',
        expectedCounts = 'data/auxiliary/'+config['input_folder']+'/kmers/{kmer}/{id}/expected_counts.json',
        groundTruthFile = 'data/input/' + config['ground_truth']
    output:
        differences = 'data/output/'+config['input_folder']+'/methodAnalysis/{id}/{kmer}/differences_observed_expected.txt'
    params:
        inputFileID = lambda wildcards: wildcards.id
    conda:
        '../envs/biopythonworkbench.yaml'
    script:
        '../scripts/compareKmerCounts_expected.py'


rule makeSequenceProfiles:
    input:
        sequences = 'data/auxiliary/spaSequences.fa'
    output:
        profiles = 'data/auxiliary/kmers/{kmer}/spaSequences.kmerprofiles.json',
        counts = 'data/auxiliary/kmers/{kmer}/spaSequences.counts.json'
    params:
        k = lambda wildcards: wildcards.kmer,
        # cluster execution
        cpus = '1',
        mem = '32G',
        gpus = '0',
        walltime = '00:30:00'
    conda:
        '../envs/biopythonworkbench.yaml'
    script:
        '../scripts/makeKmerProfiles.py'


rule calcAverageReadLength:
    input:
        read1 = 'data/input/'+config['input_folder']+'/{id}'+config['input_read_1_ending']
    output:
        'data/auxiliary/'+config['input_folder']+'/{id}/readLengthEstimate.txt'
    conda:
        '../envs/biopythonworkbench.yaml'
    shell:
        'awk \' {{ if(NR%4==2) {{count++; bases += length}} }} END{{print bases/count}} \' {input.read1}  > {output}' #todo: use both read files?
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rule createSpaTypeVennDiagram:
    input:
        expected = 'data/auxiliary/'+config['input_folder']+'/kmers/{kmer}/{id}/expected_counts.json',
        observed = 'data/auxiliary/'+config['input_folder']+'/kmers/{kmer}/{id}/alignment.counts.json',
        scores = 'data/auxiliary/'+config['input_folder']+'/kmers/{kmer}/{id}/scores.probabilistic_cov.tsv'
    output:
        venngtt = 'data/output/'+config['input_folder']+'/kmers/{kmer}/{id}/spaTypesGroundTruthVennDia.svg',
        venntopsix = 'data/output/'+config['input_folder']+'/kmers/{kmer}/{id}/spaTypesTopSixVennDia.svg',
        vennrandomsix = 'data/output/'+config['input_folder']+'/kmers/{kmer}/{id}/spaTypesRandomSixVennDia.svg'
    params:
        gtt = lambda wildcards : getGroundTruthType(wildcards.id)
    log:
        'logs/'+config['input_folder']+'/probabilistic/kmers/{kmer}/{id}/spaTypeVennDia.log'
    conda:
        '../envs/biopythonworkbench.yaml'
    script:
        '../scripts/spaTypeVennDia.py'
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rule createValidKmerHistogram:
    input:
        expected = 'data/auxiliary/'+config['input_folder']+'/kmers/{kmer}/{id}/expected_counts.json',
        observed = 'data/auxiliary/'+config['input_folder']+'/kmers/{kmer}/{id}/alignment.counts.json'
    output:
        histogram = 'data/auxiliary/'+config['input_folder']+'/kmers/{kmer}/{id}/validKmersHisto.svg'
    params:
        gtt = lambda wildcards : getGroundTruthType(wildcards.id)
        
    log:
        'logs/'+config['input_folder']+'/probabilistic/kmers/{kmer}/{id}/validKmersHisto.log'
    conda:
        '../envs/biopythonworkbench.yaml'
    script:
        '../scripts/createValidKmerHistogram.py'


rule calcKmerErrorRates:
    input:
        baseError = 'data/auxiliary/'+config['input_folder']+'/{id}/base_error_estimate.txt'
    output:
        error = 'data/auxiliary/'+config['input_folder']+'/kmers/{kmer}/{id}/error_estimate.txt'
    params:
        k = lambda wildcards: wildcards.kmer
    log:
        'logs/'+config['input_folder']+'/kmers/{kmer}/{id}/calcKmerErrorRates.log'
    conda:
        '../envs/biopythonworkbench.yaml'
    script:
        '../scripts/calcKmerErrorRate.py'


if config['skipMapping']:
    rule makeReadProfiles:
        input:
            expectedCounts = 'data/auxiliary/'+config['input_folder']+'/kmers/{kmer}/{id}/expected_counts.json',
            read1 = 'data/auxiliary/' + config['input_folder'] + '/{id}' + '.qc' + config['input_read_1_ending'],
            read2 = 'data/auxiliary/' + config['input_folder'] + '/{id}' + '.qc' + config['input_read_2_ending']
        output:
            profile = 'data/auxiliary/'+config['input_folder']+'/kmers/{kmer}/{id}/alignment.profile.json',
            counts = 'data/auxiliary/'+config['input_folder']+'/kmers/{kmer}/{id}/alignment.counts.json'
        params:
            k = lambda wildcards: wildcards.kmer,
            #cluster execution
            cpus = '1',
            mem = '32G',
            gpus = '0',
            walltime = '00:30:00'
        log:
            'logs/'+config['input_folder']+'/kmers/{kmer}/{id}/makeReadProfiles.log'
        conda:
            '../envs/biopythonworkbench.yaml'
        script:
            '../scripts/makeKmerProfilesFromFastq.py'

else:			
    rule makeReadProfiles:
        input:
            alignment = 'data/auxiliary/'+config['input_folder']+'/{id}/alignment.filtered.bam',
            read1 = 'data/auxiliary/' + config['input_folder'] + '/{id}' + '.qc' + config['input_read_1_ending'],
            read2 = 'data/auxiliary/' + config['input_folder'] + '/{id}' + '.qc' + config['input_read_2_ending'],
            index = 'data/auxiliary/'+config['input_folder']+'/{id}/alignment.filtered.bam.bai', #ghost input
            regions = 'data/auxiliary/syntheticProteinAs.meta'
        output:
            profile = 'data/auxiliary/'+config['input_folder']+'/kmers/{kmer}/{id}/alignment.profile.json',
            counts = 'data/auxiliary/'+config['input_folder']+'/kmers/{kmer}/{id}/alignment.counts.json',
            debug = 'data/auxiliary/'+config['input_folder']+'/kmers/{kmer}/{id}/alignmentExtraction.txt'
            #local_coverage_estimate = 'data/auxiliary/kmers/{kmer}/{id}/local_coverage_estimate.txt'
        params:
            k = lambda wildcards: wildcards.kmer,
            #cluster execution
            cpus = '1',
            mem = '32G',
            gpus = '0',
            walltime = '00:30:00'
        log:
            'logs/'+config['input_folder']+'/kmers/{kmer}/{id}/makeReadProfiles.log'
        conda:
            '../envs/biopythonworkbench.yaml'
        script:
            '../scripts/makeKmerProfileFromSam.py'
        

rule createRatios:
    input:
        'data/auxiliary/kmers/{kmer}/spaSequences.counts.json'
    output:
        'data/auxiliary/kmers/{kmer}/spaSequencesRatios.json'
    params:
        k = lambda wildcards: wildcards.kmer,
        #cluster execution
        cpus = '1',
        mem = '8G',
        gpus = '0',
        walltime = '00:15:00'
    conda:
        '../envs/biopythonworkbench.yaml'
    script:
        '../scripts/calculateKmerRatios.py'

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rule createDistanceMatrixOverKmersOfV:
    input:
        kmers = 'data/auxiliary/kmers/{kmer}/spaSequences.counts.json'
    output:
        V_kmers_distances = 'data/auxiliary/kmers/{kmer}/V_kmers.distances.npz'
    params:
        k = lambda wildcards: wildcards.kmer,
        hamming_distance_cutoff = 5,
        #cluster execution
        cpus = '2',
        mem = '4G',
        gpus = '0',
        walltime = '00:15:00'
    conda:
        '../envs/biopythonworkbench.yaml'
    script:
        '../scripts/calcDistanceMatrixVkmers.py'